Plasmid

Part:BBa_K4212035:Design

Designed by: Fontaine Gibbs   Group: iGEM22_Imperial_College_London   (2022-09-30)


ChiS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1903
    Illegal NheI site found at 1011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1903
    Illegal BamHI site found at 1888
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1903
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists the fusion protein consisted of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. We added a flexible linker to this construct to test if it works better than the construct without the linker.


Source

Synthetic construct.

References

https://parts.igem.org/Part:BBa_K143015