Plasmid

Part:BBa_K4212034:Design

Designed by: Fontaine Gibbs   Group: iGEM22_Imperial_College_London   (2022-09-30)


ChiS1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1867
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1867
    Illegal NheI site found at 1011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1867
    Illegal BamHI site found at 1852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1867
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1867
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists the chimera of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong terminator, which helps to increase the production of desired protein.

Source

Synthetic Construct.

References

https://parts.igem.org/Part:BBa_K143015