Plasmid_Backbone

Part:BBa_K4212029

Designed by: Yuancheng Ding   Group: iGEM22_Imperial_College_London   (2022-09-30)


STK-1D-sfGFP

Destination plasmid containing an sfGFP dropout

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1102
    Illegal XbaI site found at 1117
    Illegal PstI site found at 2180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1102
    Illegal PstI site found at 2180
    Illegal NotI site found at 1108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1102
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1102
    Plasmid lacks a suffix.
    Illegal PstI site found at 2180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1102
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1117
    Illegal PstI site found at 2180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2158
    Illegal BsaI.rc site found at 1140
    Illegal SapI.rc site found at 1324


[edit]
Categories
Parameters
None