Plasmid_Backbone

Part:BBa_K4212028:Design

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-10-03)


STK-1B-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
    Illegal XbaI site found at 1067
    Illegal PstI site found at 2130
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
    Illegal PstI site found at 2130
    Illegal NotI site found at 1058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1052
    Plasmid lacks a suffix.
    Illegal PstI site found at 2130
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1052
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1067
    Illegal PstI site found at 2130
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2108
    Illegal BsaI.rc site found at 1090
    Illegal SapI.rc site found at 1274


Design Notes

sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. Dropout cassette is replaced by target sequences, which enables to verify the insertion of target sequences if transformants do not express sfGFP.


Source

Synthetic construct.

References