Coding
Part:BBa_K4212006:Design
Designed by: Shirin Bamezai Group: iGEM22_Imperial_College_London (2022-09-29)
D15
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 52
Illegal PstI site found at 812 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 812
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 52
Illegal PstI site found at 812 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 52
Illegal PstI site found at 812 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for B. subtilis.
Source
Protein sequence from T5 bacteriophage genome, coding sequence optimised for B. subtilis.