Coding

Part:BBa_K4212006:Design

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)


D15


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 52
    Illegal PstI site found at 812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 812
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 52
    Illegal PstI site found at 812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 52
    Illegal PstI site found at 812
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for B. subtilis.


Source

Protein sequence from T5 bacteriophage genome, coding sequence optimised for B. subtilis.

References