Coding

Part:BBa_K4212005:Design

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)


gRNA_SDP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Composed of a gRNA scaffold and target region aimed at origin of replication of BBa_K4212031 part (Golden Gate level 2 B. subtilis expression vector).


Source

Synthetic construct

References