Coding
CenA

Part:BBa_K4211017:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-08-19)


CenA: Endoglucanase A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • No BioBrick restriction enzyme cut sites in coding region.
  • Constructed by PCR.
  • We added a 6xHis tag at the end of the gene to purify the protein. To terminate transcription, we added the TAA sequence.

Forward primer

5'- GCT CCC GGC TGC CGC -3'

Reverse primer

5'- TAT TAA TAA CTA TCA GTG ATG GTG ATG GTG ATG CCA CCT GGC GTT GCG -3'

Source

The source of this section is a sequence retrieved from GenBank.

References

Din, N. et al. The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding. Mol Microbiol 11, 747-755 (1994). https://doi.org:10.1111/j.1365-2958.1994.tb00352.x