Coding
Cex

Part:BBa_K4211016:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-08-19)


Cex: Exoglucanase/xylanase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • No BioBrick restriction enzyme cut sites in coding region.
  • Constructed by PCR.
  • We added a 6xHis tag at the end of the gene to purify the protein. To terminate transcription, we added the TAA sequence.

Forward primer

5'- GCG ACC ACG CTC AAG GAG G -3'

Reverse primer

5'- TAT TAA TAA CTA TTA GTG ATG GTG ATG GTG ATG GCC GAC CGT GCA GG -3'

Source

The source of this section is a sequence retrieved from GenBank.

References

Ong, E., Gilkes, N. R., Miller, R. C., Jr., Warren, R. A. & Kilburn, D. G. The cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas fimi: production in Escherichia coli and characterization of the polypeptide. Biotechnol Bioeng 42, 401-409 (1993). https://doi.org:10.1002/bit.260420402