Composite
sigIL-LL-3

Part:BBa_K4211003:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-08-19)


LL-37 secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • No BioBrick restriction enzyme cut sites in coding region.
  • Constructed by PCR.

Forward primer

5'- CGC GGA TCC ATG TAC AGG ATG CAA CTC CTG T -3'

Reverse primer

5'- TTT ATA GCG GCC GCT TAG GAC TCT GTC CTG GGT ACA AG -3'

Source

The BNUZH-China iGEM team 2022 designed and built.

References

[1] Wang, G. et al. Design of Antimicrobial Peptides: Progress Made with Human Cathelicidin LL-37. Adv Exp Med Biol 1117, 215-240 (2019). https://doi.org:10.1007/978-981-13-3588-4_12
[2] Stecher, C., Maurer, K. P., Kastner, M. T. & Steininger, C. Human Cytomegalovirus Induces Vitamin-D Resistance In Vitro by Dysregulating the Transcriptional Repressor Snail. Viruses 14 (2022). https://doi.org:10.3390/v14092004
[3] Chessa, C. et al. Antiviral Effect of hBD-3 and LL-37 during Human Primary Keratinocyte Infection with West Nile Virus. Viruses 14 (2022). https://doi.org:10.3390/v14071552