Measurement

Part:BBa_K4204016:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-08)


Crystal violet induced sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 456
    Illegal PstI site found at 792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 907
    Illegal NheI site found at 1005
    Illegal PstI site found at 456
    Illegal PstI site found at 792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 907
    Illegal BglII site found at 916
    Illegal XhoI site found at 1728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 456
    Illegal PstI site found at 792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 456
    Illegal PstI site found at 792
    Illegal NgoMIV site found at 477
    Illegal AgeI site found at 327
    Illegal AgeI site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There must not have any gap between the RBS and the start codon in this circuit. Gaps may cause lower transcriptional activity, or even shift of open reading frame.


Source

The crystal violet induction system is from Ruegg et al.'s work [1]. Assembled by BNDS_China 2022.

References

[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3