RNA
Part:BBa_K4204006:Design
Designed by: Ziyue Lei Group: iGEM22_BNDS_China (2022-10-05)
The trigger of ProQC system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.
Source
The design of the trigger is inspired by the eucaryotic translation system.
References
[1]Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.