Regulatory

Part:BBa_K4204004:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-09-26)


Transcriptional unit of EilR.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is designed to replace the promotor, RBS, and ORF of the original repressor. The terminator is added artificially later. Also, the promoter of the sequence might be shorter than marked, since the original research does not mark the promoter range clearly.

Source

From research done by Ruegg et al [1]. Synthesized by twist bioscience.

References

[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3

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