Regulatory

Part:BBa_K4204004

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-09-26)


Transcriptional unit of EilR.

Crystal violet induction system is a cheap, efficient and sensitive induction system. The repressor protein EilR could bind to operator eilOc and inhibit transcription when crystal violet is absent. Its working principle is shown in the figure below.

This part is the whole gene circuit of EilR, the repressor of the crystal violet induction system. This sequence contains a promoter, RBS, and the coding sequence of eilR, and a strong terminator.

For detailed characterization,see BBa_K4204016

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
    Illegal AgeI site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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