Other
Part:BBa_K4204003:Design
Designed by: Jianfei Song Group: iGEM22_BNDS_China (2022-10-04)
Transcriptional unit of sgRNA targeting HBLA gene of B.cereus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 26
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 26
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The last 20 bp is the target region and could be replaced with other sequences that pairs with the target gene.
Source
Designed by BNDS_China 2022, based on [1]'s paper.
References
[1] Li, L., Li, S., Wu, N., Wu, J., Wang, G., Zhao, G., & Wang, J. (2019). HOLMESv2: a CRISPR-Cas12b-assisted platform for nucleic acid detection and DNA methylation quantitation. American Chemical Society Synthetic Biology, 8(10), 2228–2237. https://doi.org/10.1021/acssynbio.9b00209