Generator

Part:BBa_K4202045:Design

Designed by: Zhijian Yan, Zhuoyun Ren   Group: iGEM22_ZJU-China   (2022-10-03)


Sucrose regulated kill switch in Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 926
    Illegal AgeI site found at 654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1139


Design Notes

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Source

The original sequence of PsacB is obtained from subtiwiki (http://subtiwiki.uni-goettingen.de/v4/gene?id=AAA944BE7F01CE90F7730EECA16F3E4ED77D165A).The sequence of PsacB is obtained from genomic DNA of Bacillus subtilis through PCR. The original sequence of mazF is obtained from NCBI (https://www.ncbi.nlm.nih.gov/gene/947252). The original sequence of mazE is obtained from NCBI (https://www.ncbi.nlm.nih.gov/gene/947245). The codon optimization of mazE and mazF were both carried out using the online tool developed by Genescript and the optimized sequence is synthesized by Genescript, too.

References