Measurement

Part:BBa_K4202033:Design

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-10-03)


Pveg-1x leader RNA-spoVG RBS-GFP for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111


Design Notes

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Source

The original sequence of leader RNA is obtained from subtiwiki (http://subtiwiki.uni-goettingen.de/v4/gene?id=AAA944BE7F01CE90F7730EECA16F3E4ED77D165A). The sequence of leader RNA is obtained from genomic DNA of Bacillus subtilis through PCR.The original sequence of Pveg and spoVG RBS are both obtained from BBa_K1364008(https://parts.igem.org/Part:BBa_K1364008). The original sequence of GFP is obtained from BBa_E0040(https://parts.igem.org/wiki/index.php?title=Part:BBa_E0040).

References