Regulatory

Part:BBa_K4202027:Experience

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-10-03)


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Applications of BBa_K4202027

Characterization


Vector construct

To characterization this part, we constructed the measurement device BBa_K4202035 and then constructed it to the pHY300PLK plasmid. The vector was then transformed into Bacillus subtilis WB600 strain.


Characterization method

The transformant were inoculate to 2ml LB medium and cultured in the test tube overnight. The next day, the culture were inoculated into 2ml TB medium containing 0%, 0.1%, 0.5%, 1%, 2%, 5% sucrose (w/v) at the ratio of 1:100. After cultured in 37℃, 220rpm for 7 hours, the culture were diluted into PBS at the ratio of 1:1000 and were measured the fluorescence intensity through flow cytometry. The cluster of Bacillus subtilis should be easily gated from SSC-A vs. FSH-H figure.


Gate.jpg
Fig 1 The Bacillus subtilis in the SSC-A vs. FSH-H figure

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