Coding
Part:BBa_K4202015:Design
Designed by: ZIHANG YE Group: iGEM22_ZJU-China (2022-09-30)
This part is a fusion protein of EutM and SpyCatcher , and it`s used for the bio-scafford
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to ensure the fucntion of two motives of the fusion protein, a GS linker was utilized for the connection of them.
Source
The main sequence of EutM(GenBank: EDB1220699.1) and the synthetic constructed SpyCatcher(GenBank: AFD50637.1) are obtained from the NCBI. We optimized the codon of the sequence for the protein expression in bacillus subtilis