Coding
Part:BBa_K4202010:Design
Designed by: Hongying LIN Group: iGEM22_ZJU-China (2022-09-30)
Amorphous Calcium CarbonateBinding Protein(ACCBP)(without the 16 amino acids at N terminus)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 45
Illegal AgeI site found at 308 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 514
Design Notes
BBa_K4202010 is a part of ACCBP without signal sequence.Besides,to test the effect of N-terminal amino acid sequence on protein folding,we delete 16 amino acids at N terminus.
Source
The sequence of this part was originally retrieved from GenBank DQ473430.1. The codons of this sequence was optimized by Genscipt.