Coding
Hag-SpyT
Part:BBa_K4202006:Design
Designed by: ZIHANG YE Group: iGEM22_ZJU-China (2022-09-25)
This part is a fusion protein of Hag and SpyTag , and it`s used for the bio-scafford.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 568
Design Notes
The proper site should be chosen for the insertion of SpyTag.
Source
We obtain the sequence of Hag from the <a herf="http://subtiwiki.uni-goettingen.de/">subtiwiki</a> and design the fusion protein based on the former research.The plasmid was synthesized by GenScript.