Coding

Part:BBa_K4202003:Design

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-09-25)


This part is a fusion protein of mRFP and a engineered protein named SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein possesses one tag (6*His), two major motifs. To ensure the correct spatial structure of the fusion proteins, several non-polar amino acids (G and S) were used to link the different motifs


Source

The sequence of these two proteins are obtained from NCBI. And the plasmid was synthesized by GenScript Company.

References