Coding

Part:BBa_K4202002:Design

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-09-25)


This part is a fusion protein of mRFP and a short peptide named SpyTag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 391
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 391
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 391
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein possesses one tag (HA), two major motifs. To ensure the correct spatial structure of the fusion proteins, several non-polar amino acids (G and S) were used to link the different motifs

Source

The seuqence of mRFP and SpyTag are obtained from NCBI. And the plasmid is synthesized by GenScrip company.

References