Coding
Part:BBa_K4202001:Design
Designed by: Zhijian Yan Group: iGEM22_ZJU-China (2022-09-24)
mazF(codon optimization for Bacillus subtilis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 158
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 371
Design Notes
The codon was optimized to get better expression in Bacillus subtilis.
Source
The original sequence is obtained from NCBI (https://www.ncbi.nlm.nih.gov/gene/947252). The codon optimization was carried out using the online tool developed by Genescript and the optimized sequence is synthesized by Genescript, too.