Coding

Part:BBa_K4202001:Design

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-09-24)


mazF(codon optimization for Bacillus subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 158
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 371


Design Notes

The codon was optimized to get better expression in Bacillus subtilis.


Source

The original sequence is obtained from NCBI (https://www.ncbi.nlm.nih.gov/gene/947252). The codon optimization was carried out using the online tool developed by Genescript and the optimized sequence is synthesized by Genescript, too.

References