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Regulatory
Gmubi
Part:BBa_K4201000:Design
Designed by: Maya Li Nelson Group: iGEM22_CU-Boulder (2022-10-01)
Glycine max Polyubiquitin Promoter (Gmubi)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 790
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 934
Design Notes
This promoter is native to G. max1 and it is best if used in G. max transgenic constructs.
Source
Glycine max Polyubiquitin Promoter (Gmubi) is sourced from the genome of the common soybean, G. max.
References
1. De La Torre, C. M. & Finer, J. J. The intron and 5′ distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues. Plant Cell Rep. 34, 111–120 (2015).