Regulatory
Gmubi

Part:BBa_K4201000:Design

Designed by: Maya Li Nelson   Group: iGEM22_CU-Boulder   (2022-10-01)


Glycine max Polyubiquitin Promoter (Gmubi)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 934


Design Notes

This promoter is native to G. max1 and it is best if used in G. max transgenic constructs.


Source

Glycine max Polyubiquitin Promoter (Gmubi) is sourced from the genome of the common soybean, G. max.

References

1. De La Torre, C. M. & Finer, J. J. The intron and 5′ distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues. Plant Cell Rep. 34, 111–120 (2015).