Part:BBa_K4195180

Biology

This sequence is a conserved region of toxin gene pirB (1). It’s used as the detection target of RENDR system.
Ribozyme ENabled Detection of RNA (RENDR)
RENDR is a high-performing, plug-and-play RNA-sensing platform. RENDR utilizes a split variant of the Tetrahymena thermophila ribozyme by synthetically splitting it into two non-functional fragments. Two fragments are each appended with designed RNA guide sequences, which can interact with the RNA input of interest. The split ribozyme is then inserted within a desired gene output. When binded with the RNA input, two transcribed split ribozyme fragments are triggered to self-splice and thus the intact transcript of the protein output will form.

Fig. 1 Schematic illustration of RENDR.

Usage and Design

This part is used as the target of the detection system BBa_K4195187、BBa_K4195188、BBa_K4195189 and BBa_K4195190. We build the circuit similar as BBa_K4195178.

Reference

1. J. M. S. Lazarte et al. Passive Immunization with Recombinant Antibody VLRB-PirA(vp)/PirB(vp)-Enriched Feeds against Vibrio parahaemolyticus Infection in Litopenaeus vannamei Shrimp. Vaccines (Basel). 9, (2021).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 207
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]