Device

Part:BBa_K4194012:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


p(TetR)-RBS-EGFP-LVA-RBS-δ activator-LVA-terminator-pF-RBS-mRFP1-LVA-RBS-TetR-LVA-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1387
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 961
    Illegal NotI site found at 2105
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1387
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1387
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 1056
    Illegal NgoMIV site found at 1071
    Illegal AgeI site found at 1314
    Illegal AgeI site found at 1987
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Escherichia coli has been done.


Source

None

References