Composite

Part:BBa_K4194010:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


p(TetR)-RBS-GFP-LVA-RBS-δ activator-LVA-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 943
    Illegal NgoMIV site found at 1038
    Illegal NgoMIV site found at 1053
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding region has been optimized for codons in Escherichia coli.


Source

None

References