Coding

Part:BBa_K4194004:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


pleD(+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 458
    Illegal NotI site found at 607
    Illegal NotI site found at 710
    Illegal NotI site found at 1357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization has been performed for Escherichia coli.


Source

Caulobacter crescentus pleD gene.

References