Coding

Part:BBa_K4194002:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


LacI-LVA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 913
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 913
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 913
    Illegal NgoMIV site found at 429
    Illegal AgeI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization has been performed for Escherichia coli.


Source

LacI come from Escherichia coli lactose operon.

References