Composite

Part:BBa_K4193031:Design

Designed by: Xinchen Yue   Group: iGEM22_OUC-China   (2022-10-08)


kill switch 1-L2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
    Illegal PstI site found at 729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
    Illegal PstI site found at 729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 669
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
    Illegal PstI site found at 729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
    Illegal PstI site found at 729
    Illegal AgeI site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the kill switch contains YopE (cytotoxin form Yersinia) mRNA and a ribozyme riboswitch (contained a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence). When there is target molecule binds to the aptamer sequence, YopE is expressed and kills the cell, whereas the hammerhead ribozyme sequence degrades YopE mRNA.


Source

synthesize from company


References

Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.