Coding

Part:BBa_K4193018:Design

Designed by: Rui Shi   Group: iGEM22_OUC-China   (2022-09-29)


PTP2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1214
    Illegal EcoRI site found at 1539
    Illegal SpeI site found at 1051
    Illegal PstI site found at 1301
    Illegal PstI site found at 2000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1214
    Illegal EcoRI site found at 1539
    Illegal SpeI site found at 1051
    Illegal PstI site found at 1301
    Illegal PstI site found at 2000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1214
    Illegal EcoRI site found at 1539
    Illegal BglII site found at 255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1214
    Illegal EcoRI site found at 1539
    Illegal SpeI site found at 1051
    Illegal PstI site found at 1301
    Illegal PstI site found at 2000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1214
    Illegal EcoRI site found at 1539
    Illegal SpeI site found at 1051
    Illegal PstI site found at 1301
    Illegal PstI site found at 2000
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

an endogenous Hog1 protein phosphatase

Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.