Regulatory

Part:BBa_K4193012:Design

Designed by: Rui Shi   Group: iGEM22_OUC-China   (2022-09-29)


kill switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
    Illegal PstI site found at 698
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
    Illegal PstI site found at 698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 661
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
    Illegal PstI site found at 698
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
    Illegal PstI site found at 698
    Illegal AgeI site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the kill switch contains YopE mRNA, which is a cytotoxin form Yersinia, and a ribozyme riboswitch. When no target molecule binds to the aptamer sequence, YopE is expressed and kills the cell, whereas the hammerhead ribozyme sequence degrades YopE mRNA.

Source

synthesize from company

References

Win, M.N. and Smolke, C.D. (2007). A modular and extensible RNA-based gene-regulatory platform for engineering cellular function. Proceedings of the National Academy of Sciences, 104(36), pp.14283–14288. doi:10.1073/pnas.0703961104.