Composite

Part:BBa_K4192143:Design

Designed by: Jiang Nanqi   Group: iGEM22_CAU_China   (2022-09-30)


PcysJ-PcysH-mazE-mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction was unsuccessful, and PcysH was skipped directly in gibson assembly to construct PcysJ-mazE-mazF, probably due to the homologous arm design


Source

All individual components were derived from E. coli.

References

[1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1).