Composite

Part:BBa_K4192112:Design

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)


Plac-RBS-gacA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 521
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part need more test because its effect is not stable.


Source

Plac comes from plasmid pBBR1MCS-2. The rbs sequence (BBa_K4192000) is synthetic. The gacA comes from the genome of Pseudomonas fluorescens 2P24.

References