![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4192110:Design
Designed by: Lehan Dong Group: iGEM22_CAU_China (2022-09-29)
cdg-gacA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal BamHI site found at 259
Illegal XhoI site found at 357
Illegal XhoI site found at 801 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 192
Illegal NgoMIV site found at 1289
Illegal AgeI site found at 759 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 210
Illegal BsaI.rc site found at 777
Design Notes
Before using this part, please add appropriate promoters upstream, such as lac promoter,
Source
The cdg and gacA come from Pseudomonas fluorescens 2P24. The rbs sequence (BBa_K4192000) is synthetic.