Regulatory

Part:BBa_K4192040

Designed by: Jiang Nanqi   Group: iGEM22_CAU_China   (2022-09-29)


PcysJ

Novel threonine sensing promoters including PcysJ and PcysH were discovered by proteomic analyses of Escherichia coli in response to extracellular threonine challenges.

In E. coli, PcysJ was reported to be controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-l-serine.

Inducible activity of PcysJ and PcysH.png

Fig.1 Inducible activity of PcysJ and PcysH[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1).


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