Part:BBa_K419020:Experience

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Applications of BBa_K419020

Verification of the Part BBa_K419020

The DNA fragment of nhaA promoter was amplified from the part BBa_K116002 by PCR method. The nhaA PCR products (274 bp) were purified and digested with EcoRI and PstI, then ligated with EcoRI-PstI cut pSB1C3, the iGEM 2010 standard backbone. We got the pSB1C3-nhaA plasmid successfully. Furthermore, the Lux I gene with ribosome binding site (RBS) DNA was amplified from the part BBa_C0161. The Lux I PCR products (585 bp) were digested with Xba I and Pst I, then ligated with the Spe I-Pst I treated pSB1C3-nhaAvector. After E. coli transformation and plasmid preparation, the part BBa_K419020 for sensing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the nhaA-Lux I DNA fragment (859 bp). The sensing system composite was transformed into E. coli for further experiments.

User Reviews

UNIQcbc71c37ee32fdf5-partinfo-00000000-QINU UNIQcbc71c37ee32fdf5-partinfo-00000001-QINU