Plasmid
Part:BBa_K4190023:Design
Designed by: Zoe L. Petroff, Neo Peng Group: iGEM22_UCSC (2022-09-22)
mg-int-trp1-hyg_1432-4a:Ex4
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 10
Illegal SpeI site found at 1469
Illegal PstI site found at 1478 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 3263
Illegal SpeI site found at 1469
Illegal PstI site found at 1478
Illegal NotI site found at 3554
Illegal NotI site found at 5317 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BglII site found at 707
Illegal XhoI site found at 1059 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 10
Illegal SpeI site found at 1469
Illegal PstI site found at 1478 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 10
Illegal SpeI site found at 1469
Illegal PstI site found at 1478
Illegal NgoMIV site found at 1394
Illegal NgoMIV site found at 3293
Illegal AgeI site found at 323
Illegal AgeI site found at 2212 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 827