Coding
Part:BBa_K4190015:Design
Designed by: Zoe L. Petroff, Neo Peng Group: iGEM22_UCSC (2022-09-21)
EBP10 Half-life Extender
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was codon optimized for usage in S. cerevisiae.
Source
The protein sequence was originally found in the paper “In-vivo half-life and hypoglycemic bioactivity of a fusion protein of exenatide and elastin-based polypeptide from recombinant Saccharomyces cerevisiae” [1].
References
[1] S. J. Jung et al., “In-vivo half-life and hypoglycemic bioactivity of a fusion protein of exenatide and elastin-based polypeptide from recombinant Saccharomyces cerevisiae,” J Biotechnol, vol. 303, pp. 16–24, Sep. 2019, doi:10.1016/j.jbiotec.2019.06.304.