Plasmid

Part:BBa_K4190000:Design

Designed by: N/A   Group: iGEM22_UCSC   (2022-09-19)


pET28:GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 959
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 1025
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 959
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 959
    Illegal NgoMIV site found at 1057
    Illegal NgoMIV site found at 2645
    Illegal NgoMIV site found at 2805
    Illegal NgoMIV site found at 5852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3725


Design Notes

Since this space in the plasmid is flanked by a promoter and a terminator, we did not need to add these parts through Golden Gate Assembly.

Source

This part was sourced from addgene.