Plasmid
Part:BBa_K4188005:Design
Designed by: Tobias Bröker Group: iGEM22_WWU_Muenster (2022-09-28)
pSB3CY_Ura shuttle vector with uracile synthase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6236
Illegal XbaI site found at 3425
Illegal SpeI site found at 3790
Illegal PstI site found at 1105 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6236
Illegal NheI site found at 4922
Illegal SpeI site found at 3790
Illegal PstI site found at 1105
Illegal NotI site found at 4863 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6236
Illegal BamHI site found at 2441 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6236
Illegal XbaI site found at 3425
Illegal SpeI site found at 3790
Illegal PstI site found at 1105 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6236
Illegal XbaI site found at 3425
Illegal SpeI site found at 3790
Illegal PstI site found at 1105
Illegal AgeI site found at 793
Illegal AgeI site found at 905
Illegal AgeI site found at 2083
Illegal AgeI site found at 2406 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1378
Illegal BsaI site found at 6242
Illegal BsaI.rc site found at 1099
Illegal SapI site found at 1082
Illegal SapI.rc site found at 6
Design Notes
For more information about the design of this vector, you can visit the page BBa_K4188001.
Source
The auxotrophy has already been added to the ready-to-use shuttle vectors to make their use as easy and as fast as possible. For more information on the origin, plasmid visit the part BBa_K4188001.