Plasmid

Part:BBa_K4181002:Design

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-09-18)


A shuttle plasmid that functions in Bacillus subtilis and Escherichia coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal XbaI site found at 2810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal NheI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal BglII site found at 2877
    Illegal BglII site found at 5958
    Illegal BamHI site found at 2804
    Illegal XhoI site found at 5962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal XbaI site found at 2810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal XbaI site found at 2810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6853
    Illegal SapI.rc site found at 4030
    Illegal SapI.rc site found at 7935


Design Notes

This plasmid has only one strong promoter, after which the target gene needs to be inserted


Source

This is an artificially constructed vector plasmid

References