Plasmid
Part:BBa_K4181002:Design
Designed by: Junzhi Yan Group: iGEM22_SJTU-BioX-Shanghai (2022-09-18)
A shuttle plasmid that functions in Bacillus subtilis and Escherichia coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal XbaI site found at 2810 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal NheI site found at 1092 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal BglII site found at 2877
Illegal BglII site found at 5958
Illegal BamHI site found at 2804
Illegal XhoI site found at 5962 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal XbaI site found at 2810 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1256
Illegal EcoRI site found at 3406
Illegal XbaI site found at 2810 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6853
Illegal SapI.rc site found at 4030
Illegal SapI.rc site found at 7935
Design Notes
This plasmid has only one strong promoter, after which the target gene needs to be inserted
Source
This is an artificially constructed vector plasmid