Plasmid

Part:BBa_K4181002

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-09-18)


A shuttle plasmid that functions in Bacillus subtilis and Escherichia coli

This plasmid, named PHT01, is an expression vector for COTE-binding protein and DSUP protein that we need to express. It can be introduced into Escherichia coli and then introduced into Bacillus subtilis for expression

Plasmid profiles

2022-SJTU-Biox-Shanghai-12.png

Figure 1.PDG1730

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal XbaI site found at 2810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal NheI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal BglII site found at 2877
    Illegal BglII site found at 5958
    Illegal BamHI site found at 2804
    Illegal XhoI site found at 5962
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal XbaI site found at 2810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1256
    Illegal EcoRI site found at 3406
    Illegal XbaI site found at 2810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6853
    Illegal SapI.rc site found at 4030
    Illegal SapI.rc site found at 7935


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