Coding
ChimeraII

Part:BBa_K4179012:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-10-05)


PT1a with the donor docking domain switched to the domain of G4DT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 634
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1213
    Illegal PstI site found at 634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 699
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 634
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 634
    Illegal NgoMIV site found at 739
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

• This gene was codon optimized for E. coli.


Source

Each AA sequence was taken from Uniprot[1] and codon optimized for E. coli.

References

1. Bateman, Alex, et al. “Uniprot: The Universal Protein Knowledgebase in 2021.” Nucleic Acids Research, vol. 49, no. D1, 2020, https://doi.org/10.1093/nar/gkaa1100.