Composite

Part:BBa_K4179003:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-10-05)


PcPT under Rhlr promoter + P2A + mCherry reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 703
    Illegal EcoRI site found at 1066
    Illegal XbaI site found at 2146
    Illegal PstI site found at 226
    Illegal PstI site found at 589
    Illegal PstI site found at 1786
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 703
    Illegal EcoRI site found at 1066
    Illegal NheI site found at 1351
    Illegal PstI site found at 226
    Illegal PstI site found at 589
    Illegal PstI site found at 1786
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 703
    Illegal EcoRI site found at 1066
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 703
    Illegal EcoRI site found at 1066
    Illegal XbaI site found at 2146
    Illegal PstI site found at 226
    Illegal PstI site found at 589
    Illegal PstI site found at 1786
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 703
    Illegal EcoRI site found at 1066
    Illegal XbaI site found at 2146
    Illegal PstI site found at 226
    Illegal PstI site found at 589
    Illegal PstI site found at 1786
    Illegal AgeI site found at 1425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.