Composite

Part:BBa_K4175055:Design

Designed by: Chentao Li   Group: iGEM22_ZJUintl-China   (2022-10-07)


CAR-COSMO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1227
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1227
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1227
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1227
    Illegal NgoMIV site found at 899
    Illegal NgoMIV site found at 1692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 444
    Illegal BsaI.rc site found at 732


Design Notes

We inserted the COSMO sequence between the 4-1BB and CD3ζ sequences in the hope that COSMO could block the signal for 4-1BB activation while the COSMO sequence could activate CD3ζ downstream of it when caffeine was present.



Source

COSMO sequence is dexcribed in BBa_K4175052.


References