Coding

Part:BBa_K4175012:Design

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-09-25)


UAS-pSV40-CAR19-P2A-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1388
    Illegal NgoMIV site found at 1803
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 949


Design Notes

The sequence has been codon optimized for human.


Source

UAS-pSV40 (BBa_K4040033), CD8a signal peptide (BBa_K4040014), anti-CD19 (BBa_K2549005), CD8 hinge domain (BBa_K4175004), CD8 transmembrane domain (BBa_K4175005), 4-1BB (BBa_K4175006), CD3-ζ domain (BBa_K4175009)

References