Coding
Part:BBa_K4175000:Design
Designed by: Li Xianxiu, Zhang Wanying Group: iGEM22_ZJUintl-China (2022-08-27)
anti-IL-6 scFv
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 604
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 604
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 512
Illegal XhoI site found at 496 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 604
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 604
Illegal AgeI site found at 556 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence has been condon optimized for human.
Source
Human. Sequence found in ADRIAN, T.H.J., DARIO, C., 2021. Neutralization of Human Cytokines with Membrane-Bound Anti-Cytokine Non-Signaling Binders Expressed in Immune Cells. https://www.lens.org/lens/patent/040-383-008-445-12X/citations?view=in
References
Tan, A.H.J., Vinanica, N., Campana, D., 2020. Chimeric antigen receptor–T cells with cytokine neutralizing capacity. Blood Adv. 4, 1419–1431. https://doi.org/10.1182/bloodadvances.2019001287