Composite

Part:BBa_K4171024:Design

Designed by: HUANG, YEN-JUNG   Group: iGEM22_NCKU_Tainan   (2022-08-16)


NPS-tyrP-Ptrc-melA-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2786
    Illegal AgeI site found at 338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2638
    Illegal BsaI.rc site found at 1943


Design Notes

None


Source

tyrP: BBa_K2997000

Trc promoter: BBa_K864400

melA: BBa_K274001

References

Wang Z, Tschirhart T, Schultzhaus Z, et al. Melanin Produced by the Fast-Growing Marine Bacterium Vibrio natriegens through Heterologous Biosynthesis: Characterization and Application. Applied and Environmental Microbiology. 2020;86(5):e02749-19. doi:10.1128/AEM.02749-19