Device

Part:BBa_K417007:Design

Designed by: Ben Porebski, Andrew Perry   Group: iGEM10_Monash_Australia   (2010-10-26)


Simple inducible (LacI) ethylene generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 912
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Native LacI promoter (BBa_R0010) was chosen since it facilitates easier assembly compared with the shorter alternative BBa_R0011, although it would be worth testing both these promoters. It is unknown if the order of the coding sequences is important in this device.


Source

See part documentation for enzyme coding sequences BBa_K417001 and BBa_K417002 for details on the origin of the sequences.

References